Antimicrobial & Free Radical Scavenging Activities of Green Synthesised Copper Nanoparticles from Musa acuminata and Citrus sinensis Peel Extracts
Published 2022-12-26
Keywords
- Green synthesis, Copper nanoparticles, Peel extract, Antimicrobial property, Scavenging activity.
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Abstract
The biosynthesis of nanoparticles is a kind of bottom-up approach where the main reaction occurring is reduction/oxidation. Re-cently, green synthesis of nanoparticles using plant extracts has gained more attention, since they are simple, cost-effective, non-toxic, environment friendly and easily scaled up for large-scale synthesis. There is a great demand for synthesizing Copper nano-particles (CuNPs) by simple and less expensive methods. The peels of a variety of fruits have gained attention as a natural source of antioxidants. Green synthesis of CuNPs was successfully obtained from bio-reduction of copper sulfate pentahydrate solutions using <i>Musa acuminata</i> and <i>Citrus sinensis</i>. CuNPs have been appropriately characterized using UV-vis spectroscopy and SEM analysis. It was observed that CuNPs synthesized from dried orange peel extract had better anti-microbial and antioxidant activi-ty than the fresh orange peel and showed great activity than all the other three samples. Since copper is well-known for its anti-microbial properties, we assumed that fabricating CuNP from banana and orange peel extract can increase its efficiency. The antimicrobial activity of the nanoparticles was analyzed using gram-positive (<i>Staphylococcus aureus</i>), gram-negative (<i>Escherichia coli</i>), and fungal (<i>Aspergillus niger</i>) species. Due to their benign and stable nature and antimicrobial property, these CuNPs may be well utilized for industrial and medicinal purposes.
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References
- Aminuzzaman M, Kei LM, Liang WH. (2017). Green synthesis of copper oxide (CuO) nanoparticles using banana peel extract and their photocatalytic activities. AIP Conference Proceed-ings.1828(1). DOI: https://doi.org/10.1063/1.4979387
- Ananda Murthy HC, Desalegn T, Kassa M, Abebe B, Assefa T. (2020). Synthesis of Green Copper Nanoparticles Using Medic-inal Plant Hagenia abyssinica (Brace) JF. Gmel. Leaf Extract: Antimicrobial Properties. J. Nanomater. 1 – 12. DOI: https://doi.org/10.1155/2020/3924081
- Arul Dhas N, Paul Raj C, Gedanken A. (1988). Synthesis, Characterization, and Properties of Metallic Copper Nanopar-ticles. Chem. Mater. 10(5): 1446 - 1452. DOI: https://doi.org/10.1021/cm9708269
- Arya A, Gupta K, Chundawat TS, Vaya D. (2018). Biogenic synthesis of copper and silver nanoparticles using green al-ga Botryococcus braunii and its antimicrobial activi-ty. Bioinorg. Chem. Appl. DOI: https://doi.org/10.1155/2018/7879403
- Begum NA, Mondal S, Basu S, Laskar RA, Mandal D. (2009). Biogenic synthesis of Au and Ag nanoparticles using aqueous solutions of Black Tea leaf extracts. Colloids Surf B Biointer-faces. 71(1):113 - 8. DOI: https://doi.org/10.1016/j.colsurfb.2009.01.012
- Chatterjee AK, Chakraborty R, Basu T. (2014). Mechanism of antibacterial activity of copper nanoparticles. Nanotechnology. 25(13). DOI: https://doi.org/10.1088/0957-4484/25/13/135101
- Din MI, Arshad F, Hussain Z, Mukhtar M. (2017). Green adeptness in the synthesis and stabilization of copper nano-particles: catalytic, antibacterial, cytotoxicity, and antioxidant activities. Nanoscale Res. Lett. 12(1). DOI: https://doi.org/10.1186/s11671-017-2399-8
- Dixit S, Tiwari S. (2008). Impact assessment of heavy metal pollution of Shahpura Lake, Bhopal, India. Int. J. Environ. Res. 2(1): 37-42.
- Esau SR, Roberto SB, Ocotlan-Flores J, Saniger JM. (2010). Synthesis of AgNPs by sonochemical induced reduction appli-cation in SERS. J. Nanopart. Res. 9:77. DOI: https://doi.org/10.4028/www.scientific.net/JNanoR.9.77
- Jung T, Höhn A, Catalgol B, Grune T. (2009). Age-related dif-ferences in oxidative protein-damage in young and senescent fibroblasts. Arch. Biochem. Biophys. 1;483(1):127-35. DOI: https://doi.org/10.1016/j.abb.2008.12.007
- Khalid H, Shamaila S, Zafar N. (2015). Synthesis of copper nanoparticles by chemical reduction method. Sci. Int. 27(4): 3085-3088.
- Khanna PK, Gaikwad SM, Adhyapak PV, Singh NK, Marimu-thu R. (2007). Synthesis and characterization of copper nano-particles. Materials Letters, 61: 4711- 4714. DOI: https://doi.org/10.1016/j.matlet.2007.03.014
- Lalitha A, Subbaiya R, Ponmurugan P. (2013). Green synthesis of silver nanoparticles from leaf extract Azhadirachta indica and to study its anti-bacterial and antioxidant activity. Int. J. Curr. Microbiol. App. Sci. 2(6): 228 - 235.
- Muthusamy S, Selvam K, Rajamani R. (2014). Synthesis of Pomegranate Peel Extract Mediated Silver Nanoparticles and its Antibacterial Activity. Am. J. Adv. Drug Deliv. 2(2): 174 – 182.
- Nidhi N, Santosh K, Ghosh T, Dutta PK. (2009). Preparation of Chitosan based silver nano composites by a facile method. International Conference on Optics and Photonics. Chandigarh: CSIO.
- Packer L, Cadenas E, Davies KJA. (2008). Free radicals and exercise: An introduction. Free Radic. Biol. Med. 44(2):123-5. DOI: https://doi.org/10.1016/j.freeradbiomed.2007.05.031
- Padam BS, Tin HS, Chye FY, Abdullah MI. (2014). Banana by-products: an under-utilized renewable food biomass with great potential. J. Food Sci. Technol. 51(12): 3527-45. DOI: https://doi.org/10.1007/s13197-012-0861-2
- Ren G, Meng Y, Zhang Q, Tang M, Zhu B, Chai F, Wang C, Su Z. (2018). Nitrogen-doped carbon dots for the detection of mercury ions in living cells and visualization of latent finger-prints. New J. Chem. 42: 6824 - 6830. DOI: https://doi.org/10.1039/C7NJ05170K
- Ruch RJ, Cheng SJ, Klaunig JE. (1989). Prevention of cytotoxi-city and inhibition of intracellular communication by antioxi-dant catechins isolated from Chinese green tea. Carcinogene-sis. 10(6):1003-8. DOI: https://doi.org/10.1093/carcin/10.6.1003
- Shindume LH, Zhao Z, Wang N, Liu H, Umar A, Zhang J, Wu T, Guo Z. (2019). Enhanced photocatalytic activity of B, N-codoped TiO2 by a new molten nitrate process. J. Nanosci. Nanotechnol. 19: 839 - 849. DOI: https://doi.org/10.1166/jnn.2019.15745
- Sreeram KJ, Nidhin M, Nair BU. (2008). Microwave assisted template synthesis of silver nanoparticles. Bull. Mater. Sci. 31(7): 937 – 942. DOI: https://doi.org/10.1007/s12034-008-0149-3