Volume 3, Issue 1, June 2023
Original Research

Production and Characterization of Polyhydroxy butyrate from Pseudomonas aeruginosa

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  • Jesvin Pixy,
  • Rakhi Raju,
  • Elcey C. Daniel
Jesvin Pixy
Biotechnology Research Division, Department of Life Sciences, Kristu Jayanti College, Autonomus, Bengaluru, Karnataka-560077
Rakhi Raju
Biotechnology Research Division, Department of Life Sciences, Kristu Jayanti College, Autonomus, Bengaluru, Karnataka-560077
Elcey C. Daniel
Biotechnology Research Division, Department of Life Sciences, Kristu Jayanti College, Autonomus, Bengaluru, Karnataka-560077
DOI: https://doi.org/10.59176/kjcab.v3i1.2362

Published 2024-07-08

Keywords

  • PHB, wheat bran, corn cob powder, coconut husk, FTIR

How to Cite

Pixy, J., Raju, R., & Daniel, E. C. (2024). Production and Characterization of Polyhydroxy butyrate from <i>Pseudomonas aeruginosa</i>. Kristu Jayanti Journal of Core and Applied Biology (KJCAB), 3(1), 1–9. https://doi.org/10.59176/kjcab.v3i1.2362
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Abstract

Poly(3-hydroxybutyrate) (PHB) synthesis and accumulationis a mobilizable carbon repository of certain bacteria tothrive the carbon limitation. It is a common carbon- andenergy-storage compound, degrades into its monomer 3-hydroxybutyrate (3HB) or D-β-hydroxybutyric acid (DBD)with in the cell under the microaerobic condition. A soilbacterial strain accumulating PHB was isolated andidentified as Pseudomonas aeruginosa by 16S rRNA genesequencing (NCBI accession number MF062071). The organism was subjected to PHB production under varyingfactors to establish the laboratory scale production withsubstrate preferences of economic choice. Among thesubstrates, wheat bran favored a higher PHB production, 0.9 g/l, compared to corn cob powder and coconut husk. The dry cell biomass was also maximum with thesubstrate, wheat bran. Neutral to near neutral pH (7.0) andan ambient temperature (37°C) showed as ideal underaerobic conditions. The PHB accumulated was extracted,purified and was subjected to FT/IR spectroscopy. A majorpeak apart from other peaks and a strong absorption bandat 1651.83cm-1 corresponding to the C=O thioester bondreveal the functional group which confirm the presence of intracellular PHB.

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