Volume 3, Issue 2, December 2023
Original Research

Assessment of Anti-Bacterial Property of Selected Essential Oils, Lemongrass, Lemon, Eucalyptus, Citronella, and Peppermint against Escherichia coli DH5α Strain

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  • Rishika Sai Yerramalli,
  • Aksa Saji,
  • Abhishek Singh,
  • Grace K Davis,
  • Nibeditha Pradhan,
  • Priya Josson Akkara
Rishika Sai Yerramalli
Department of Life Sciences, Kristu Jayanti College Autonomous, Bengaluru-560077, Karnataka
Aksa Saji
Department of Life Sciences, Kristu Jayanti College Autonomous, Bengaluru-560077, Karnataka
Abhishek Singh
Department of Life Sciences, Kristu Jayanti College Autonomous, Bengaluru-560077, Karnataka
Grace K Davis
Department of Life Sciences, Kristu Jayanti College Autonomous, Bengaluru-560077, Karnataka
Nibeditha Pradhan
Department of Life Sciences, Kristu Jayanti College Autonomous, Bengaluru-560077, Karnataka
Priya Josson Akkara
Department of Life Sciences, Kristu Jayanti College Autonomous, Bengaluru-560077, Karnataka
DOI: https://doi.org/10.59176/kjcab.v3i2.2383

Published 2024-08-14

Keywords

  • Essential oils, Antibacterial Activity, <i>Escherichia coli</i>, Lemon

How to Cite

Yerramalli, R. S., Saji, A. ., Singh, A. ., Davis, G. K., Pradhan, N., & Akkara, P. J. (2024). Assessment of Anti-Bacterial Property of Selected Essential Oils, Lemongrass, Lemon, Eucalyptus, Citronella, and Peppermint against <i>Escherichia coli</i> DH5α Strain. Kristu Jayanti Journal of Core and Applied Biology (KJCAB), 3(2), 51–58. https://doi.org/10.59176/kjcab.v3i2.2383
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Abstract

Essential oils (EOs) have been used as a common home cure for numerous illnesses, including headaches, anxiety, and insomnia, since ancient times. They are concentrated hydrophobic liquids that are rich in a complex variety of terpenoid hydrocarbons and contain secondary metabolites of plants. The objective of this study was to have a comparative analysis of the antibacterial characteristics of five widely used essential oils: lemongrass, lemon, eucalyptus, citronella, and peppermint. Escherichia coli DH5α strain was chosen because of its quick growth and ease of maintenance. The Agar well diffusion method was used to conduct the microbiological assay. Measure the antibacterial effectiveness of the oils, zones of inhibition were assessed. The current study's findings demonstrate that each of the five essential oils used has discernible antibacterial action, although the most potent impact is found in lemon essential oil. Further studies can be done to find the effect of a mixture of different essential oils in different proportions and the mechanism of its action.

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References

  1. Afshar, M. K., Moghadam, Z. B., Taghizadeh, Z., Bekhradi,R., Montazeri, A., & Mokhtari, P. (2015). Lavender fragrance essential oil and the quality of sleep in postpartum women. Iran Red Crescent Med J, 17(4)https://doi.org/10.5812/ircmj.17(4)2015.25880
  2. Boukhatem, M. N., Ferhat, M., Kameli, A., Saidi, F., & Kebir,H. T. (2014). Lemon grass (Cymbopogon citratus) essential oil as a potent anti-inflammatory and antifungal drugs. Libyan J Med, 9(1),25431https://doi.org/10.3402/ljm.v9.25431
  3. Carson, C., Mee, B., & Riley, T. V. (2002). Mechanism of Action of Melaleuca alternifolia (Tea Tree) Oil on Staphylococcus aureus Determined by Time-Kill, Lysis,Leakage, and Salt Tolerance Assays and ElectronMicroscopy. Antimicrob Agents Chemother, 46(6), 1914–1920 https://doi.org/10.1128/aac.46.6.1914-1920.2002
  4. Chao, S., Young, D. G., & Oberg, C. J. (2000). Screeningfor inhibitory activity of essential oils on selected bacteria,fungi and viruses. J Essent Oil Res, 12(5), 639–649 https://doi.org/10.1080/10412905.2000.9712177
  5. De Sousa, D. P., De Almeida Soares Hocayen, P., Andrade, L. N., & Andreatini, R. (2015). A Systematic review of the Anxiolytic-Like Effects of Essential oils in animal models. Molecules, 20(10), 18620–18660.https://doi.org/10.3390/molecules201018620
  6. García‐Márquez, J., Bárany, A., Ruiz, Á. B., Costas, B.,Arijo, S., & Mancera, J. M. (2021). Antimicrobial and Toxic Activity of Citronella Essential Oil (Cymbopogon nardus),and Its Effect on the Growth and Metabolism of Gilthead Seabream (Sparus aurata L.). Fishes, 6(4), 61.https://doi.org/10.3390/fishes6040061
  7. Göbel, H., Fresenius, J., Heinze, A., Dworschak, M., & Soyka, D. (1996). Effektivität von Oleum menthaepiperitae und von Paracetamol in der Therapie des Kopfschmerzes vom Spannungstyp. Der Nervenarzt,67(8), 672–681. https://doi.org/10.1007/s001150050040
  8. Kalemba, D., & Kunicka, A. (2003). Antibacterial and antifungal properties of essential oils. Curr Med Chem,10(10), 813–829 https://doi.org/10.2174/0929867033457719
  9. Kumar, A., Agarwal, K., Maurya, A. K., Shanker, K.,Bushra, U., Tandon, S., & Bawankule, D. U. (2015).Pharmacological and phytochemical evaluation of Ocimum sanctum root extracts for its antiinflammatory, analgesic and antipyretic activities. Pharmacogn Mag,11(42), 217 https://doi.org/10.4103/0973-1296.157743
  10. Lillehei, A. S., & Halcón, L. L. (2014). A systematic review of the effect of inhaled essential oils on sleep. JAltern Complement Med, 20(6), 441–451.https://doi.org/10.1089/acm.2013.0311
  11. Mahato, N., Sharma, K., Koteswararao, R., Sinha, M.,Baral, E., & Cho, M. H. (2017). Citrus essential oils:Extraction, authentication, and application in food preservation. Crit Rev Food Sci Nutr, 59(4), 611–625https://doi.org/10.1080/10408398.2017.1384716
  12. Mahmud, F., Mahedi, M. R. A., Afrin, S., Haque, R.,Hasan, M. S., Sum, F. A., Bary, M. A., Syrmos, N., &Kuri, O. C. (2022). Biological & Insecticidal Effect of Citronella Oil: A Short Review. Clin Med Health Res J,2(6), 261–265 https://doi.org/10.18535/cmhrj.v2i6.108
  13. Nostro, A., Roccaro, A. S., Bisignano, C., Marino, A.,Cannatelli, M., Pizzimenti, F., Cioni, P. L., Procopio, F.,& Blanco, A. R. (2007). Effects of oregano, carvacroland thymol on Staphylococcus aureus and Staphylococcus epidermidis biofilms. J Med Microbiol,56(4), 519–523. https://doi.org/10.1099/jmm.0.46804-0
  14. Prabu seenivasan, S., Jayakumar, M., & Ignaci muthu, S.(2006). In vitro antibacterial activity of some plant essential oils. BMC Complement Altern Med, 6(1)https://doi.org/10.1186/1472-6882-6-39
  15. Schweitzer, B., Balázs, V. L., Molnár, S., Szögi-Tatár, B.,Böszörményi, A., Palkovics, T., Horváth, G., &Schneider, G. (2022). Antibacterial Effect of Lemongrass (Cymbopogon citratus) against the Aetiological Agents of Pitted Keratolyis. Molecules,27(4), 1423. https://doi.org/10.3390/molecules27041423
  16. Sienkiewicz, M., Łysakowska, M., Pastuszka, M.,Bienias, W., & Kowalczyk, E. (2013). The potential of use Basil and rosemary essential oils as effective antibacterial agents. Molecules, 18(8), 9334–9351. https://doi.org/10.3390/molecules18089334
  17. Sowndhararajan, K., Kim, M., Deepa, P., & Park, S. J. (2018). Application of the P300 Event-Related Potential in the Diagnosis of Epilepsy Disorder: A review. Sci Pharm, 86(2), 10 https://doi.org/10.3390/Scipharm86020010
  18. Steflitsch, W., Steiner, D., Peinhaupt, W., Riedler, B., Smuc, M.,& Diewald, G. (2015). Gesundheits förderung durch Stress- und Burnout-Prophylaxe mit ätherischen Ölen für alle Berufsgruppen im Wiener Otto-Wagner-Spital. Complement Med Res, 22(3),185–194. https://doi.org/10.1159/000433619
  19. Ünalan, I., Slavik, B., Buettner, A., Goldmann, W. H., Frank, G.,& Boccaccını, A. R. (2019). Physical and Antibacterial Properties of Peppermint Essential Oil Loaded Poly (ε-caprolactone) (PCL) Electrospun Fiber Mats for Wound Healing. Front Bioeng Biotechnol,https://doi.org/10.3389/fbioe.2019.00346
  20. Verma, R. S., Verma, S. K., Tandon, S., Padalia, R. C., & Darokar, M. P. (2020). Chemical composition and antimicrobial activity of Java citronella (Cymbopogon winterianus Jowitt exBor) essential oil extracted by different methods. J Essent OilRes,32(5),449–455https://doi.org/10.1080/10412905.2020.1787885